Recombinant humaan EF-1 delta/EEF1D-eiwit

Eukaryotic translation elongation factor 1 delta, also known as EEF1D, is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Overexpression of EEF1D is associated with oesophageal carcinoma and may adversely affect the outcome of medulloblastomas. Recombinant human EEF1D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Eukaryotic translation elongation factor 1 delta, EF-1D, EF1D, Elongation factor 1-delta, Antigen NY-CO-4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.2 kDa (301aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

EEF1D, Eukaryotic translation elongation factor 1 delta, EF-1D, EF1D, ATGP0955-10 µg, ATGP0955-20 µg, ATGP0955-50 µg, ATGP0955-100 µg, ATGP0955-250 µg, ATGP0955-500 µg, ATGP0955-1 mg, ATGP0955-10, ATGP0955-20, ATGP0955-50, ATGP0955-100, ATGP0955-250, ATGP0955-500, ATGP0955-1

De Bortoli M., et al. (2006) BMC Cancer. 6:223.; ; Cans C., et al. (2003) Proc Natl Acad Sci. 100:13892-13897.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_001951

P29692

Human

MGSSHHHHHH SSGLVPRGSH MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVAKSSILL DVKPWDDETD MAQLEACVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I