Recombinant humaan EF-1 gamma/EEF1G-eiwit

Elongation factor 1-gamma, also known as EEF1G, is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. Increased expression of EEF1G is associated with pancreatic cancer, suggesting a possible role for EEF1G in the oncogenic transformation process. Recombinant human EEF1G protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Elongation factor 1-gamma, EF1G, GIG35, EF-1-gamma, Elongation factor 1 gamma, GIG35, PRO1608

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

52.5 kDa (460aa)

EEF1G, Elongation factor 1-gamma, EF1G, GIG35, EF-1-gamma, Elongation factor 1 gamma, GIG35, PRO1608, ATGP2763-10 µg, ATGP2763-20 µg, ATGP2763-50 µg, ATGP2763-100 µg, ATGP2763-250 µg, ATGP2763-500 µg, ATGP2763-1 mg, ATGP2763-10, ATGP2763-20, ATGP2763-50, ATGP2763-100, ATGP2763-250, ATGP2763-500, ATGP2763-1

Sanders J., et al. (1992) Nucleic Aciads Res. 20: 5907-5910.; ; Koonin E V., et al. (1994) Protein Sci. 3: 2045-2054.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_001395

P26641

Human

MAAGTLY TYPENWRAFK ALIAAQYSGA QVRVLSAPPH FHFGQTNRTP EFLRKFPAGK VPAFEGDDGF CVFESNAIAY YVSNEELRGS TPEAAAQVVQ WVSFADSDIV PPASTWVFPT LGIMHHNKQA TENAKEEVRR ILGLLDAYLK TRTFLVGERV TLADITVVCT LLWLYKQVLE PSFRQAFPNT NRWFLTCINQ PQFRAVLGEV KLCEKMAQFD AKKFAETQPK KDTPRKEKGS REEKQKPQAE RKEEKKAAAP APEEEMDECE QALAAEPKAK DPFAHLPKST FVLDEFKRKY SNEDTLSVAL PYFWEHFDKD GWSLWYSEYR FPEELTQTFM SCNLITGMFQ RLDKLRKNAF ASVILFGTNN SSSISGVWVF RGQELAFPLS PDWQVDYESY TWRKLDPGSE ETQTLVREYF SWEGAFQHVG KAFNQGKIFK