Recombinant humaan EGLN3/PHD3-eiwit

EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Egl nine homolog 3, HIFPH3, PHD3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.8 kDa (263aa) confirmed by MALDI-TOF

EGLN3, PHD3, Egl nine homolog 3, HIFPH3, PHD3, ATGP1609-10 µg, ATGP1609-20 µg, ATGP1609-50 µg, ATGP1609-100 µg, ATGP1609-250 µg, ATGP1609-500 µg, ATGP1609-1 mg, ATGP1609-10, ATGP1609-20, ATGP1609-50, ATGP1609-100, ATGP1609-250, ATGP1609-500, ATGP1609-1

Epstein A.C.R., et al. (2001) Cell. 107:43-54; ; Lee S., et al. (2000) Cancer Cell. 8:155-167

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTA

Metabolism

NP_071356

Q9H6Z9

Human

MPLGHI MRLDLEKIAL EYIVPCLHEV GFCYLDNFLG EVVGDCVLER VKQLHCTGAL RDGQLAGPRA GVSKRHLRGD QITWIGGNEE GCEAISFLLS LIDRLVLYCG SRLGKYYVKE RSKAMVACYP GNGTGYVRHV DNPNGDGRCI TCIYYLNKNW DAKLHGGILR IFPEGKSFIA DVEPIFDRLL FFWSDRRNPH EVQPSYATRY AMTVWYFDAE ERAEAKKKFR NLTRKTESAL TED