Recombinant humaan eIF-1A/EIF1-eiwit

EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sui1iso1, SuI1, Protein translation factor SUI1 homolog, ISO1, Eukaryotic translation initiation factor 1, EIF1A, EIF-1, EIF1, A121

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

16.9 kDa (150aa) confirmed by MALDI-TOF

Sui1iso1, SuI1, Protein translation factor SUI1 homolog, ISO1, Eukaryotic translation initiation factor 1, EIF1A, EIF-1, EIF1, A121, ATGP0461-10 µg, ATGP0461-20 µg, ATGP0461-50 µg, ATGP0461-100 µg, ATGP0461-250 µg, ATGP0461-500 µg, ATGP0461-1 mg, ATGP0461-010, ATGP0461-020, ATGP0461-050, ATGP0461-100, ATGP0461-250, ATGP0461-500, ATGP0461-01M

Dennis MD., et al. (2009) J Biol Chem. 284 (31) :20615-28. ; ; Pisarev AV., et al. (2007) Cell. 131 (2) :286-99.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol

Epigenomics (Transcription & Translation)

NP_005792

P41567

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF