Recombinant humaan eIF-1AY/EIF1 AY-eiwit

EIF1AY is similar to eukaryotic translation initiation factor 1A (EIF1A) . This protein seems to be required for maximal rate of protein biosynthesis. It enhances ribosome dissociation into subunits and is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. Recombinant human EIF1AY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Eukaryotic translation initiation factor 1A Y-linked, Eukaryotic translation initiation factor 1A, Y-linked, eIF-1A Y isoform, eukaryotic translation initiation factor 4C (eIF-4C)

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

18.8 kDa (167aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

EIF1AY, Eukaryotic translation initiation factor 1A Y-linked, Eukaryotic translation initiation factor 1A, Y-linked, eIF-1A Y isoform, eukaryotic translation initiation factor 4C (eIF-4C), ATGP0902-10 µg, ATGP0902-20 µg, ATGP0902-50 µg, ATGP0902-100 µg, ATGP0902-250 µg, ATGP0902-500 µg, ATGP0902-1 mg, ATGP0902-10, ATGP0902-20, ATGP0902-50, ATGP0902-100, ATGP0902-250, ATGP0902-500, ATGP0902-1

Mahbybyl Huq AH., et al. (2009) J Child Neurol. 24 (10) :1258-61; ; Page DC., et al (1997) Science. 278 (5338) :675-80.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 100 mM NaCl, 20% glycerol

Epigenomics (Transcription & Translation)

NP_004672

O14602

Human

MPKNKGK GGKNRRRGKN ENESEKRELV FKEDGQEYAQ VIKMLGNGRL EALCFDGVKR LCHIRGKLRK KVWINTSDII LVGLRDYQDN KADVILKYNA DEARSLKAYG ELPEHAKINE TDTFGPGDDD EIQFDDIGDD DEDIDDI