Recombinant humaan eIF-1b/EIF1B-eiwit

EIF1B is crucial for the scanning process in vitro. During the scanning process, EIF1B is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. This protein enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. Recombinant human EIF1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Eukaryotic translation initiation factor 1B, GC20, Protein translation factor SUI1 homolog GC20

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

15 kDa (133aa) confirmed by MALDI-TOF

EIF1B, Eukaryotic translation initiation factor 1B, GC20, ATGP0963-10 µg, ATGP0963-20 µg, ATGP0963-50 µg, ATGP0963-100 µg, ATGP0963-250 µg, ATGP0963-500 µg, ATGP0963-1 mg, ATGP0963-10, ATGP0963-20, ATGP0963-50, ATGP0963-100, ATGP0963-250, ATGP0963-500, ATGP0963-1

Pestova TV., et al. (2002) Genes Dev. 16 (22) :2906-22. ; ; Asano K., et al. (2000) Genes Dec. 14 (19) :2534-46.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol, 2mM DTT, 0.1M NaCl

Signal Transduction

NP_005866

O60739

Human

MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF