Recombinant humaan eIF-2B alfa/EIF2B1-eiwit

EIF2B1 is one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Phosphorylation of eIF2 inhibits GEF activity of EIF2B, an inhibition that requires the eIF2B1 subunit. Defects in eIF2B1 are a cause of leukoencephalopathy with vanishing white matter (VWM), a brain disease that is characterized by head trauma and motor deterioration. Recombinant human EIF2B1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Eukaryotic translation initiation factor 2B subunit alpha, Eukaryotic translation initiation factor 2B subunit 1 alpha 26kDa, EIF-2Balpha, EIF-2B, EIF2BA, EIF2Balpha, Translation initiation factor eIF-2B subunit alpha, eIF-2B GDP-GTP exchange factor subunit alpha

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.8 kDa (325aa) confirmed by MALDI-TOF

EIF2B1, Eukaryotic translation initiation factor 2B subunit 1 alpha 26kD, Eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kD, EIF-2B, EIF-2Balpha, EIF2B, EIF2BA, MGC117409, MGC125868, MGC125869, ATGP1024-10 µg, ATGP1024-20 µg, ATGP1024-50 µg, ATGP1024-100 µg, ATGP1024-250 µg, ATGP1024-500 µg, ATGP1024-1 mg, ATGP1024-10, ATGP1024-20, ATGP1024-50, ATGP1024-100, ATGP1024-250, ATGP1024-500, ATGP1024-1

Van der Knaap M.S. et al. (2002) Ann. Neurol 51: 264-270 ; ; Mohammad-Qureshi S.S. et al. (2007) Mol. Cell. Biol. 27: 5225-5234.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Development

NP_001405.1

Q14232

Human

MDDKELIEYF KSQMKEDPDM ASAVAAIRTL LEFLKRDKGE TIQGLRANLT SAIETLCGVD SSVAVSSGGE LFLRFISLAS LEYSDYSKCK KIMIERGELF LRRISLSRNK IADLCHTFIK DGATILTHAY SRVVLRVLEA AVAAKKRFSV YVTESQPDLS GKKMAKALCH LNVPVTVVLD AAVGYIMEKA DLVIVGAEGV VENGGIINKI GTNQMAVCAK AQNKPFYVVA ESFKFVRLFP LNQQDVPDKF KYKADTLKVA QTGQDLKEEH PWVDYTAPSL ITLLFTDLGV LTPSAVSDEL IKLYL