Recombinant humaan eIF-3 bèta/EIF3 I eiwit

EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3I protein, fused to His-tag at N-terminus, was expressed in E. coli.

C3orf68, eIF3-beta, eIF-3-beta, eIF3i, eIF3-p36, EIF3S2, Eukaryotic translation initiation factor 3 subunit 2, Eukaryotic translation initiation factor 3 subunit 2 beta 36kDa, Eukaryotic translation initiation factor 3 subunit I, PRO2242, TGF-beta receptor-interacting protein 1, TRIP1, TRIP-1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.9 kDa (348aa)

EIF3I, eukaryotic translation initiation factor 3 subunit I, C3orf68, ATGP2393-10 µg, ATGP2393-20 µg, ATGP2393-50 µg, ATGP2393-100 µg, ATGP2393-250 µg, ATGP2393-500 µg, ATGP2393-1 mg, ATGP2393-10, ATGP2393-20, ATGP2393-50, ATGP2393-100, ATGP2393-250, ATGP2393-500, ATGP2393-1

Proshkin, S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980; ; Perez, R.E., et al. (2011) Am. J. Physiol. Lung Cell Mol. Physiol. 300 (5), L799-L807;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Epigenomics (Transcription & Translation)

NP_003748

Q13347

Human

MGSSHHHHHH SSGLVPRGSH MGSMKPILLQ GHERSITQIK YNREGDLLFT VAKDPIVNVW YSVNGERLGT YMGHTGAVWC VDADWDTKHV LTGSADNSCR LWDCETGKQL ALLKTNSAVR TCGFDFGGNI IMFSTDKQMG YQCFVSFFDL RDPSQIDNNE PYMKIPCNDS KITSAVWGPL GECIIAGHES GELNQYSAKS GEVLVNVKEH SRQINDIQLS RDMTMFVTAS KDNTAKLFDS TTLEHQKTFR TERPVNSAAL SPNYDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFEFEFEA