Recombinant humaan eIF-3 bèta/EIF3 I eiwit

EIF3I, also known as eukaryotic translation initiation factor 3 subunit I, is a multiprotein complex that functions during the initiation phase of eukaryotic translation. This protein is one out of 13 subunits of the mammalian EIF3 complex which associated with the 40S ribosome and facilitates the formation of 43S pre-initiation complex. This complex stimulates nearly all steps of translation initiation. These factors also appear to participate in other phases of translation, such as recycling, where it promotes the splitting of post-termination ribosomes. Also, it is shown to act as a signaling transducer by bind to and activating AKT1 through preventing PP2A-induced AKT1 dephosphorylation in tumor cells. Recombinant Human EIF3I, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

C3orf68, eIF3-beta, eIF-3-beta, eIF3i, eIF3-p36, EIF3S2, Eukaryotic translation initiation factor 3 subunit 2, Eukaryotic translation initiation factor 3 subunit 2 beta 36kDa, Eukaryotic translation initiation factor 3 subunit I, PRO2242, TGF-beta receptor-interacting protein 1, TRIP1, TRIP-1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

37.3 kDa (331aa)

EIF3I, ATGP3875-10 µg, ATGP3875-20 µg, ATGP3875-50 µg, ATGP3875-100 µg, ATGP3875-250 µg, ATGP3875-500 µg, ATGP3875-1 mg, ATGP3875-10, ATGP3875-20, ATGP3875-50, ATGP3875-100, ATGP3875-250, ATGP3875-500, ATGP3875-1

Mayeur GL., et al, (2003) Eur. J. Biochem. 270:4133-4139.; ; Pan W., et al, (2017) Vet. Microbiol. 212:59-66.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 40% glycerol

Epigenomics (Transcription & Translation)

NP_003748.1

Q13347

Human

Met1