Recombinant humaan eIF-3 epsilon/EIF3 F-eiwit

EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3F protein, fused to His-tag at N-terminus, was expressed in E. coli.

Eukaryotic translation initiation factor 3 subunit F, EIF3S5, Eukaryotic translation initiation factor 3 subunit 5 epsilon 47kDa, eIF3-p47, Deubiquitinating enzyme eIF3f

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

40kDa (380aa)

Eukaryotic translation initiation factor 3, subunit F, subunit 5 epsilon 47kDa, Eukaryotic translation initiation factor 3 subunit F, EIF3S5, Eukaryotic translation initiation factor 3 subunit 5 epsilon 47kDa, eIF3-p47, Deubiquitinating enzyme eIF3f, ATGP2357-10 µg, ATGP2357-20 µg, ATGP2357-50 µg, ATGP2357-100 µg, ATGP2357-250 µg, ATGP2357-500 µg, ATGP2357-1 mg, ATGP2357-010, ATGP2357-020, ATGP2357-050, ATGP2357-100, ATGP2357-250, ATGP2357-500, ATGP2357-01M

Gaitanou M., et al (2001) . Biochem. J. 355:715-724

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Epigenomics (Transcription & Translation)

NP_003745

O00303

Human

MATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL