Recombinant humaan eIF-3K/EIF3 K-eiwit

EIF3K, also known as eukaryotic translation initiation factor 3 subunit K, belongs to the eIF3 subunit K family. It is the smallest subunit of eIF3 and it interacts with several other subunits of eIF3 and the 40S ribosomal subunit. This protein is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. It is distributed both in nucleus and cytoplasm and colocalizes with cyclin D3, a regulatory subunit of cyclin-dependent kinase 4 (Cdk4) . Recombinant human EIF3K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

PTD001, PRO1474, PLAC-24, Muscle-specific gene M9 protein, MSTP001, M9, HSPC029, Eukaryotic translation initiation factor 3 subunit K, Eukaryotic translation initiation factor 3 subunit 12, EIF3S12, EIF3-p28, eIF-3 p25, ARG134

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

27.2 kDa (238aa) confirmed by MALDI-TOF

PTD001, PRO1474, PLAC-24, PLAC24, Muscle-specific gene M9 protein, MSTP001, M9, HSPC029, Eukaryotic translation initiation factor 3 subunit K, Eukaryotic translation initiation factor 3 subunit 12, EIF3S12, EIF3-p28, EIF3-p25, eIF-3 p28, eIF-3 p25, ARG134, ATGP0972-10 µg, ATGP0972-20 µg, ATGP0972-50 µg, ATGP0972-100 µg, ATGP0972-250 µg, ATGP0972-500 µg, ATGP0972-1 mg, ATGP0972-010, ATGP0972-020, ATGP0972-050, ATGP0972-100, ATGP0972-250, ATGP0972-500, ATGP0972-01M

Mayeur G.L., et al. (2003) . Eur. J. Biochem, 270: 4133-4139.; ; Wei Z., et al. (2004) . J. Biol. Chem. 279: 34983-34990.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_037366

Q9UBQ5

Human

MGSSHHHHHH SSGLVPRGSH MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMID QAHQEERPIR QILYLGDLLE TCHFQAFWQA LDENMDLLEG ITGFEDSVRK FICHVVGITY QHIDRWLLAE MLGDLSDSQL KVWMSKYGWS ADESGQIFIC SQEESIKPKN IVEKIDFDSV SSIMASSQ