Recombinant humaan eIF-4E/EIF4E-eiwit

EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

27.2 kDa (237aa) confirmed by MALDI-TOF

EIF4E, Eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, ATGP0663-10 µg, ATGP0663-20 µg, ATGP0663-50 µg, ATGP0663-100 µg, ATGP0663-250 µg, ATGP0663-500 µg, ATGP0663-1 mg, ATGP0663-10, ATGP0663-20, ATGP0663-50, ATGP0663-100, ATGP0663-250, ATGP0663-500, ATGP0663-1

Bendahmane A., et al. (2010) PLoS One. 5 (6) :e11313.; ; Pelletier J., et al. (2010) J Gen Virol. 91 (Pt 5) :1224-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_001959.1

P06730

Human

MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV