Recombinant humaan eIF-4H/EIF4H-eiwit

Eukaryotic translation initiation factor 4H, also known as EIF4H, is a 248 amino acid protein that localizes to the perinuclear region of the cytoplasm and is expressed as two isoforms, designated short and long. EIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors EIF4A and EIF4B. EIF4H was further shown to stimulate the initial rate and extent of EIF4A-mediated mRNA secondary structure unwinding. Defects in the gene encoding EIF4H are associated with Williams- Beuren syndrome (WBS), a rare developmental disorder characterized by cardiovascular and musculo-skeletal abnormalities. Recombinant human EIF4H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Eukaryotic translation initiation factor 4H, eIF-4H, WBSCR1, WSCR1, Williams-Beuren syndrome chromosome region 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.9 kDa (272aa) confirmed by MALDI-TOF

EIF4H, Eukaryotic translation initiation factor 4H, eIF-4H, WBSCR1, WSCR1, ATGP1562-10 µg, ATGP1562-20 µg, ATGP1562-50 µg, ATGP1562-100 µg, ATGP1562-250 µg, ATGP1562-500 µg, ATGP1562-1 mg, ATGP1562-10, ATGP1562-20, ATGP1562-50, ATGP1562-100, ATGP1562-250, ATGP1562-500, ATGP1562-1

Richter Cook N J., et al. (1998) J Biol Chem. 273:7579-7587.; ; Doepker R C., et al. (2004) J Virol. 78: 4684-4699.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Epigenomics (Transcription & Translation)

NP_071496

Q15056

Human

MADFDT YDDRAYSSFG GGRGSRGSAG GHGSRSQKEL PTEPPYTAYV GNLPFNTVQG DIDAIFKDLS IRSVRLVRDK DTDKFKGFCY VEFDEVDSLK EALTYDGALL GDRSLRVDIA EGRKQDKGGF GFRKGGPDDR GMGSSRESRG GWDSRDDFNS GFRDDFLGGR GGSRPGDRRT GPPMGSRFRD GPPLRGSNMD FREPTEEERA QRPRLQLKPR TVATPLNQVA NPNSAIFGGA RPREEVVQKE QE