Recombinant humaan eIF-5A1/EIF5A-eiwit

Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer. Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques.

Eukaryotic translation initiation factor 5A-1 isoform B, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.8 kDa (154aa) confirmed by MALDI-TOF

EIF5A, Eukaryotic translation initiation factor 5A isoform B, EIF-5A, EIF5A1, eIF5AI, Eukaryotic translation initiation factor 5A isoform B eIF 4D, EIF 5A, eIF 5A 1, eIF 5A1, eIF4D, uORF, uORF A, Eukaryotic initiation factor 5A, Rev binding factor, Eukaryotic initiation factor 5A isoform 1, MGC99547, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A 1, MGC104255., ATGP0272-10 µg, ATGP0272-20 µg, ATGP0272-50 µg, ATGP0272-100 µg, ATGP0272-250 µg, ATGP0272-500 µg, ATGP0272-1 mg, ATGP0272-10, ATGP0272-20, ATGP0272-50, ATGP0272-100, ATGP0272-250, ATGP0272-500, ATGP0272-1

Saini P, et al. (2009) . Nature. 459 (7243) :118-21; ; Gosslau A., et al. (2009) . J Cell Physiol. 219 (2) :485-93;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 50 mM Tris-HCl buffer (pH 7.5) containing 10 % glycerol

Cell Cycle

NP_001961

P63241

Human

MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK