Recombinant humaan eIF-5A2/EIF5A2-eiwit

EIF5A2, a member of eukaryotic initiation factor 5A subfamily, is an essential protein tightly linked to cellular polyamine homeostasis. The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond during the initial stage of protein synthesis. It seems to be the only eukaryotic protein to have a hypusine residue, which is a post-translational modification of a lysine by the addition of a butylamino group. Recombinant human EIF5A2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

MGC124093, MGC124092, Eukaryotic translation initiation factor 5A2, Eukaryotic initiation factor 5A isoform 2, eIF5AII, EIF5A2, eIF-4D

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

18.9 kDa (173aa) confirmed by MALDI-TOF

MGC124093, MGC124092, Eukaryotic translation initiation factor 5A2, Eukaryotic initiation factor 5A isoform 2, eIF5AII, EIF5A2, EIF5A 2, eIF-4D, eIF 5A2, eIF 5A 2, ATGP0596-10 µg, ATGP0596-20 µg, ATGP0596-50 µg, ATGP0596-100 µg, ATGP0596-250 µg, ATGP0596-500 µg, ATGP0596-1 mg, ATGP0596-010, ATGP0596-020, ATGP0596-050, ATGP0596-100, ATGP0596-250, ATGP0596-500, ATGP0596-01M

Guan XY., et al. (2010) Hepatology. 51 (4) :1255-63.; ; Luk JM., et al. (2009) Int J Cancer. 127 (4) :968-76.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Epigenomics (Transcription & Translation)

NP_065123

Q9GZV4

Human

MADEIDFTTG DAGASSTYPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDYQ LICIQDGYLS LLTETGEVRE DLKLPEGELG KEIEGKYNAG EDVQVSVMCA MSEEYAVAIK PCK