Recombinant humaan eiwit Cycline B1

CCNB1, also known as cyclin B1, is a regulatory protein involved in mitosis. This protein complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Recombinant human CCNB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cyclin B1, CCNB

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

50.9 kDa (457aa)

Cyclin B1, Cyclin B1, CCNB, ATGP1612-10 µg, ATGP1612-20 µg, ATGP1612-50 µg, ATGP1612-100 µg, ATGP1612-250 µg, ATGP1612-500 µg, ATGP1612-1 mg, ATGP1612-10, ATGP1612-20, ATGP1612-50, ATGP1612-100, ATGP1612-250, ATGP1612-500, ATGP1612-1

Antinore M J., et al. (1999) . Oncogene 18 (18) :2892-900

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol

Cell Cycle

NP_114172.1

P14635

Human

MALRVT RNSKINAENK AKINMAGAKR VPTAPAATSK PGLRPRTALG DIGNKVSEQL QAKMPMKKEA KPSATGKVID KKLPKPLEKV PMLVPVPVSE PVPEPEPEPE PEPVKEEKLS PEPILVDTAS PSPMETSGCA PAEEDLCQAF SDVILAVNDV DAEDGADPNL CSEYVKDIYA YLRQLEEEQA VRPKYLLGRE VTGNMRAILI DWLVQVQMKF RLLQETMYMT VSIIDRFMQN NCVPKKMLQL VGVTAMFIAS KYEEMYPPEI GDFAFVTDNT YTKHQIRQME MKILRALNFG LGRPLPLHFL RRASKIGEVD VEQHTLAKYL MELTMLDYDM VHFPPSQIAA GAFCLALKIL DNGEWTPTLQ HYLSYTEESL LPVMQHLAKN VVMVNQGLTK HMTVKNKYAT SKHAKISTLP QLNSALVQDL AKAVAKV