Recombinant humaan eiwit Cycline B2

CCNB2, also known as G2/mitotic-specific cyclin-B2, belongs to the cyclin family. CCNB2 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF) . Recombinant human CCNB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

G2/mitotic-specific cyclin-B2, HsT17299

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

47.8 kDa (422aa) confirmed by MALDI-TOF

Cyclin B2, G2/mitotic-specific cyclin-B2, HsT17299, ATGP1583-10 µg, ATGP1583-20 µg, ATGP1583-50 µg, ATGP1583-100 µg, ATGP1583-250 µg, ATGP1583-500 µg, ATGP1583-1 mg, ATGP1583-10, ATGP1583-20, ATGP1583-50, ATGP1583-100, ATGP1583-250, ATGP1583-500, ATGP1583-1

Wang B., et al. (2008) Anal. Chem. 80:9526-9533; ; Dephoure N., et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105:10762-10767

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, 0.5M NaCl, 250 mM Imidazol

Cell Cycle

NP_004692.1

O95067

Human

HMALLRR PTVSSDLENI DTGVNSKVKS HVTIRRTVLE EIGNRVTTRA AQVAKKAQNT KVPVQPTKTT NVNKQLKPTA SVKPVQMEKL APKGPSPTPE DVSMKEENLC QAFSDALLCK IEDIDNEDWE NPQLCSDYVK DIYQYLRQLE VLQSINPHFL DGRDINGRMR AILVDWLVQV HSKFRLLQET LYMCVGIMDR FLQVQPVSRK KLQLVGITAL LLASKYEEMF SPNIEDFVYI TDNAYTSSQI REMETLILKE LKFELGRPLP LHFLRRASKA GEVDVEQHTL AKYLMELTLI DYDMVHYHPS KVAAAASCLS QKVLGQGKWN LKQQYYTGYT ENEVLEVMQH MAKNVVKVNE NLTKFIAIKN KYASSKLLKI SMIPQLNSKA VKDLASPLIG RS