Recombinant humaan EMG1-eiwit

EMG1 nucleolar protein homolog, also known as EMG1, is a 244 amino acid protein that is thought to be involved in ribosome biogenesis. Localized to the nucleolus, EMG1 participates in pre-18S rRNA processing and may play an important role in the assembly of the small ribosomal subunit, possibly controlling methylation during ribosome synthesis. A mutation in this gene has been associated with Bowen-Conradi syndrome. Recombinant human EMG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribosomal RNA small subunit methyltransferase NEP1, C2F, Grcc2f, NEP1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

29.1 kDa (267aa) confirmed by MALDI-TOF

EMG1, Ribosomal RNA small subunit methyltransferase NEP1, C2F, Grcc2f, NEP1, ATGP1617-10 µg, ATGP1617-20 µg, ATGP1617-50 µg, ATGP1617-100 µg, ATGP1617-250 µg, ATGP1617-500 µg, ATGP1617-1 mg, ATGP1617-10, ATGP1617-20, ATGP1617-50, ATGP1617-100, ATGP1617-250, ATGP1617-500, ATGP1617-1

Buchhaupt M., et al. (2001) Mol Genet Genomics. 276: 273-284; ; Liu P C., et al. (2001) Mol Biol Cell. 12: 3644-3657.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Epigenomics (Transcription & Translation)

NP_006322

Q92979

Human

MGSSHHHHHH SSGLVPRGSH MGSMAAPSDG FKPRERSGGE QAQDWDALPP KRPRLGAGNK IGGRRLIVVL EGASLETVKV GKTYELLNCD KHKSILLKNG RDPGEARPDI THQSLLMLMD SPLNRAGLLQ VYIHTQKNVL IEVNPQTRIP RTFDRFCGLM VQLLHKLSVR AADGPQKLLK VIKNPVSDHF PVGCMKVGTS FSIPVVSDVR ELVPSSDPIV FVVGAFAHGK VSVEYTEKMV SISNYPLSAA LTCAKLTTAF EEVWGVI