Recombinant humaan Enolase 1/ENO1-eiwit

Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340 nm resulting from NADH at pH 6.5 at 37C.

Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, PPH, Plasminogen-binding protein, ENO1L1, MBPB1, MPB1, ENO1-IT1, ENO1 intronic transcript 1

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340 nm resulting from NADH at pH 6.5 at 37C.

47.1 kDa (434aa) confirmed by MALDI-TOF

Tau-crystallin, Tau crystallin, PPH, Plasminogen binding protein, Phosphopyruvate hydratase, Non-Neuronal Enolase (NNE), Non-Neuronal Enolase, Non Neuronal Enolase, NNE, MYC promoter-binding protein 1, MYC promoter binding protein 1, MPB-1, MPB1, MPB 1, MBPB-1, MBPB1, MBPB 1, MBP-1, MBP1, MBP 1, Enolase-1, Enolase1, Enolase 1 (alpha) like 1, Enolase 1 (alpha), Enolase 1, ENO1L1, ENO1, EC4.2.1.11, EC 4.2.1.11, C myc promoter binding protein, Alpha enolase, 2 phospho D glycerate hydro lyase, ATGP0404-10 µg, ATGP0404-20 µg, ATGP0404-50 µg, ATGP0404-100 µg, ATGP0404-250 µg, ATGP0404-500 µg, ATGP0404-1 mg, ATGP0404-010, ATGP0404-020, ATGP0404-050, ATGP0404-100, ATGP0404-250, ATGP0404-500, ATGP0404-01M

Das R., et al. (2009) Blood. 113 (22) :5371-2.; ; ueno NT., et al. (2008) Cancer Res. 68 (22) :9302-10.;

Chen X, et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. (Oncotarget. 2017) {https://pubmed.ncbi.nlm.nih.gov/28415822/}; ; Hashimoto Y, et al. Comparison of clinical characteristics in patients with Vogt-Koyanagi-Harada disease with and without anti-retinal antibodies. (Graefes Arch Clin Exp Ophthalmol. 2019) {https://pubmed.ncbi.nlm.nih.gov/31065846/}; ; Hirakata T, et al. Acute unilateral inner retinal dysfunction with photophobia: importance of electrodiagnosis. (Jpn J Ophthalmol. 2020) {https://pubmed.ncbi.nlm.nih.gov/33180210/}; ; Hashimoto Y, et al. Comparison of clinical characteristics in patients with acute zonal occult outer retinopathy according to anti-retinal antibody status. (Graefes Arch Clin Exp Ophthalmol. 2021) {https://pubmed.ncbi.nlm.nih.gov/33876277/}; ; Inoue Y, et al. α-Enolase reduces cerebrovascular Aβ deposits by protecting Aβ amyloid formation. (Cell Mol Life Sci. 2022) {https://pubmed.ncbi.nlm.nih.gov/35916996/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol

Metabolism

NP_001419.1

P06733

Human

MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK