Recombinant humaan ENSA-eiwit

ENSA, also known as alpha endosulfine, belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein is expressed in a wide range of tissues including muscle, brain, and endocrine tissues. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SuR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. Recombinant ENSA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alpha endosulfine isoform 3, ARPP-19e, Alpha endosulfine isoform 3 Alpha endosulfine, ARPP 19e, Endosulfine alpha.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 85% by SDS-PAGE

15.5 kDa (141aa) confirmed by MALDI-TOF

ENSA, Alpha endosulfine isoform 3, ARPP-19e, Alpha endosulfine isoform 3 Alpha endosulfine, ARPP 19e, Endosulfine alpha., ATGP0457-10 µg, ATGP0457-20 µg, ATGP0457-50 µg, ATGP0457-100 µg, ATGP0457-250 µg, ATGP0457-500 µg, ATGP0457-1 mg, ATGP0457-10, ATGP0457-20, ATGP0457-50, ATGP0457-100, ATGP0457-250, ATGP0457-500, ATGP0457-1

Heron L., et al. (1998) Proc Natl Acad Sci u S A. 95 (14) :8387-91. ; ; Kim SH., et al. (2001) J Neural Transm Suppl. 61:1-9. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.2mM PMSF

Neuroscience

NP_004427

O43768

Human

MGSSHHHHHH SSGLVPRGSH MSQKQEEENP AEETGEEKQD TQEKEGILPE RAEEAKLKAK YPSLGQKPGG SDFLMKRLQK GQKYFDSGDY NMAKAKMKNK QLPSAGPDKN LVTGDHIPTP QDLPQRKSSL VTSKLAGGQV E