Recombinant humaan EphB2-eiwit

EPHB2, also known as ephrin type-B receptor 2 isoform 1, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs) that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverse signaling in the ligand-expressing cells through Ephrin. Hippocampal neurons can release vesicles containing full length EPHB2, and these are taken up by neighboring glial cells. This protein controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity. Recombinant human EPHB2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human EphB2 is coated at 2 µg/mL (100 ul/well) can bind Human Ephrin-B1 (CAT# ATGP3800) in a Functional ELISA assay.

EPH receptor B2, Ephrin type-B receptor 2, EPHB2, Developmentally-regulated Eph-related tyrosine kinase, ELK-related tyrosine kinase, EPH tyrosine kinase 3, EPH-like kinase 5, EK5, hEK5, Renal carcinoma antigen NY-REN-47, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human Ephrin-B1 (CAT# ATGP3800)

59.1 kDa (533aa)

EphB2, Ephrin type-B receptor 2 isoform 1, EPHB2, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5, ATGP3493-10 µg, ATGP3493-20 µg, ATGP3493-50 µg, ATGP3493-100 µg, ATGP3493-250 µg, ATGP3493-500 µg, ATGP3493-1 mg, ATGP3493-10, ATGP3493-20, ATGP3493-50, ATGP3493-100, ATGP3493-250, ATGP3493-500, ATGP3493-1

Pasquale EB. (2008) Cell. 133:38-52.; ; Cowan CA., et al. (2000) Neuron 26:417-430.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Cardiovascular

NP_059145

P29323

Human

Val19