Recombinant humaan Ephrine-B2-eiwit

Ephrin-B2 is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. It binds to the EPHB4 and EPHA3 receptors. Recombinant human EFNB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Ephrin B2, EFNB2, EPH-related receptor tyrosine kinase ligand 5, ligand of eph-related kinase 5, LERK-5, HTK ligand, HTK-L, EPLG5, HTKL

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.9 kDa (227aa) confirmed by MALDI-TOF

Ephrin-B2, Ephrin-B2, EPLG5, Htk-L, HTKL, LERK5, ATGP1220-10 µg, ATGP1220-20 µg, ATGP1220-50 µg, ATGP1220-100 µg, ATGP1220-250 µg, ATGP1220-500 µg, ATGP1220-1 mg, ATGP1220-10, ATGP1220-20, ATGP1220-50, ATGP1220-100, ATGP1220-250, ATGP1220-500, ATGP1220-1

Jensen, P.L. et al. (2000) Stem Cells 18: 63-6; ; Cerretti, et al. (1995) . Mol. Immunol. (ENGLAND) 32 (16) : 1197-205.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200 mM NaCl, 1mM EDTA

Neuroscience

NP_004084.1

P52799

Human

IVLEP IYWNSSNSKF LPGQGLVLYP QIGDKLDIIC PKVDSKTVGQ YEYYKVYMVD KDQADRCTIK KENTPLLNCA KPDQDIKFTI KFQEFSPNLW GLEFQKNKDY YIISTSNGSL EGLDNQEGGV CQTRAMKILM KVGQDASSAG STRNKDPTRR PELEAGTNGR SSTTSPFVKP NPGSSTDGNS AGHSGNNILG SEVALFA