Recombinant humaan Ephrine-B3-eiwit

EFNB3, also known as ephrin-B3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. It binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. Recombinant human EFNB3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Ephrin-B3, EFL6, EPLG8, LERK8, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.6 kDa (224aa)

Ephrin-B3, ATGP1658-10 µg, ATGP1658-20 µg, ATGP1658-50 µg, ATGP1658-100 µg, ATGP1658-250 µg, ATGP1658-500 µg, ATGP1658-1 mg, ATGP1658-10, ATGP1658-20, ATGP1658-50, ATGP1658-100, ATGP1658-250, ATGP1658-500, ATGP1658-1

Negrete O.A., et al. (2006) PLoS Pathog. 2:78-86; ; Brueckner K., et al. (1999) Neuron. 22:511-524

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 2M urea

Neuroscience

NP_001397

Q15768

Human

LSLEP VYWNSANKRF QAEGGYVLYP QIGDRLDLLC PRARPPGPHS SPNYEFYKLY LVGGAQGRRC EAPPAPNLLL TCDRPDLDLR FTIKFQEYSP NLWGHEFRSH HDYYIIATSD GTREGLESLQ GGVCLTRGMK VLLRVGQSPR GGAVPRKPVS EMPMERDRGA AHSLEPGKEN LPGDPTSNAT SRGAEGPLPP PSMP