Recombinant humaan Ephrine-B3-eiwit

EFNB3, also known as ephrin-B3, is a member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. EFNB3 is expressed on oligodendrocytes and neurons in the hippocampus and along the midline of the spinal cord. It is up-regulated in glioma and promotes tumor cell invasion and migration. This protein acts as the midline barrier that prevents corticospinal tract projections from recrossing when they enter the spinal gray matter. Recombinant human EFNB3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Ephrin-B3, EFL6, EPLG8, LERK8, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

23 kDa (208aa)

Ephrin-B3, Ephrin-B3, EFNB3, EFL6, EPLG8, LERK8, ATGP3499-10 µg, ATGP3499-20 µg, ATGP3499-50 µg, ATGP3499-100 µg, ATGP3499-250 µg, ATGP3499-500 µg, ATGP3499-1 mg, ATGP3499-10, ATGP3499-20, ATGP3499-50, ATGP3499-100, ATGP3499-250, ATGP3499-500, ATGP3499-1

Miao H., et al. (2009) Int J Biochem Cell Biol. 41:762-770.; ; Kullander K., et al. (2001) Genes Dev. 15:877-888.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_001397

Q15768

Human

Ala

LSLEPVY WNSANKRFQA EGGYVLYPQI GDRLDLLCPR ARPPGPHSSP NYEFYKLYLV GGAQGRRCEA PPAPNLLLTC DRPDLDLRFT IKFQEYSPNL WGHEFRSHHD YYIIATSDGT REGLESLQGG VCLTRGMKVL LRVGQSPRGG AVPRKPVSEM PMERDRGAAH SLEPGKENLP GDPTSNATSR GAEGPLPPPS MP