Recombinant humaan Epigen-eiwit

EPGN, also known as epigen isoform 1, is a novel member of the epidermal growth factor superfamily. It is the last ligand of ErbB receptors and reveals intricate relationships between affinity and mitogenicity. It stimulated the proliferation of HaCaT cells, and this proliferation was blocked by an antibody to the extracellular domain of the receptor tyrosine kinase c-erbB-1. It also may constitute a novel molecular target for wound-healing therapy with its ability to promote the growth of epithelial cells. It has been reported to have high mitogenic activity but low affinity for its receptor. It has been reported in cancer specimens of the breast, bladder, and prostate. Recombinant human EPGN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Epigen isoform 1, EPGN, ALGV3072, EPG, PRO9904

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

10.8 kDa (97aa)

Epigen, Epigen isoform 1, EPGN, ALGV3072, EPG, PRO9904, ATGP3682-10 µg, ATGP3682-20 µg, ATGP3682-50 µg, ATGP3682-100 µg, ATGP3682-250 µg, ATGP3682-500 µg, ATGP3682-1 mg, ATGP3682-10, ATGP3682-20, ATGP3682-50, ATGP3682-100, ATGP3682-250, ATGP3682-500, ATGP3682-1

Kochupurakkal BS., et al. (2005) J Biol Chem. 280:8503-8512.; ; Strachan L., et al. (2001) J Biol Chem. 276:18265-18271.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001257918

Q6UW88

Human

Ala

ADPAAVTVTP PITAQQGNWT VNKTEADNIE GPIALKFSHL CLEDHNSYCI NGACAFHHEL EKAICRCFTG YTGERCEHLT LTSYAVDSYE KHHHHHH