Recombinant humaan ErbB2/Her2-eiwit

ERBB2, also known as receptor tyrosine-protein kinase erbB-2 isoform, belongs to the epidermal growth factor (EGF) receptor family. It is an oncogene encoding a type 1 tyrosine kinase growth factor receptor. Its overexpression is associated with reduced disease free and overall survival. It is a predictor factor for poor response to chemotherapy and plays a key role in development, cell proliferation and differentiation. It is permanently coupled to phospholipase C gamma and involves tyrosine phosphorylation and activation of PLC gamma. It reflects the molecular biological properties of gastric cancer and may be clinically used to determine indications for the use of targeted drugs. It results in cellular transformation and is associated with a variety of malignancy and breast, prostate, ovarian, lung cancers. Recombinant human ERBB2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Receptor tyrosine-protein kinase erbB-2 isoform, ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

70.4 kDa (638aa)

ErbB2, Her2, Receptor tyrosine-protein kinase erbB-2 isoform, ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, ATGP3586-10 µg, ATGP3586-20 µg, ATGP3586-50 µg, ATGP3586-100 µg, ATGP3586-250 µg, ATGP3586-500 µg, ATGP3586-1 mg, ATGP3586-10, ATGP3586-20, ATGP3586-50, ATGP3586-100, ATGP3586-250, ATGP3586-500, ATGP3586-1

Hammam O., et al. (2014) J Egypt Soc Parasitol. 44:719-731.; ; Zavalishina LE., et al. (2014) Arkh Patol. 76:22-27.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cancer

NP_004439.2

P04626

Human

Thr23