Recombinant humaan ErbB3/Her3-eiwit

ERBB3, also known as receptor tyrosine-protein kinase erbB-3, is a member of the epidermal growth factor receptor (EGFR/ERBB) family of receptor tyrosine kinases. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Recombinant human ERBB3, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Receptor tyrosine-protein kinase erbB-3 isoform, c-erbB-3, ErbB-3, erbB3-S, HER3, LCCS2, MDA-BF-1, p180-ErbB3, p45-sErbB3, p85-sErbB3

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

95.6 kDa (863aa)

ErbB3, Her3, Receptor tyrosine-protein kinase erbB-3 isoform, c-erbB-3, ErbB-3, erbB3-S, HER3, LCCS2, MDA-BF-1, p180-ErbB3, p45-sErbB3, p85-sErbB3, ATGP3125-10 µg, ATGP3125-20 µg, ATGP3125-50 µg, ATGP3125-100 µg, ATGP3125-250 µg, ATGP3125-500 µg, ATGP3125-1 mg, ATGP3125-10, ATGP3125-20, ATGP3125-50, ATGP3125-100, ATGP3125-250, ATGP3125-500, ATGP3125-1

Wang S, et al (2010) Oncogene. 29 (29) :4225-36.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Apoptosis

NP_001973.2

P21860

Human