Recombinant humaan ERCC1-eiwit

ERCC1, also known as DNA excision repair protein ERCC-1, functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by uV light or formed by electrophilic compounds including cisplatin. Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) . Recombinant human ERCC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

DNA excision repair protein ERCC-1, COFS4, RAD10, uV20

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

32.5 kDa (297aa) confirmed by MALDI-TOF

ERCC1, DNA excision repair protein ERCC-1, COFS4, RAD10, uV20, ATGP1546-10 µg, ATGP1546-20 µg, ATGP1546-50 µg, ATGP1546-100 µg, ATGP1546-250 µg, ATGP1546-500 µg, ATGP1546-1 mg, ATGP1546-10, ATGP1546-20, ATGP1546-50, ATGP1546-100, ATGP1546-250, ATGP1546-500, ATGP1546-1

Van Duin M., et al. (1986) Cell. 44:913-923; ; Grimwood J., et al. (2004) Nature. 428:529-535

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Cancer

NP_001159521.1

P07992

Human

MDPGKD KEGVPQPSGP PARKKFVIPL DEDEVPPGVA KPLFRSTQSL PTVDTSAQAA PQTYAEYAIS QPLEGAGATC PTGSEPLAGE TPNQALKPGA KSNSIIVSPR QRGNPVLKFV RNVPWEFGDV IPDYVLGQST CALFLSLRYH NLHPDYIHGR LQSLGKNFAL RVLLVQVDVK DPQQALKELA KMCILADCTL ILAWSPEEAG RYLETYKAYE QKPADLLMEK LEQDFVSRSL EQLIAASRED LALCPGLGPQ KARRLFDVLH EPFLKVP