Recombinant humaan ERK1/MAPK3-eiwit

MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This protein is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Recombinant human MAPK3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, PRKM3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.2 kDa (399aa) confirmed by MALDI-TOF

PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, p44 MAPK, p44 ERK1, Mitogen-activated protein kinase 3, Mitogen activated protein kinase 3, Microtubule-associated protein 2 kinase, Microtubule associated protein 2 kinase, MAPK-3, MAPK3, MAPK 3, MAP kinase3, MAP kinase isoform p44, MAP kinase 3, Insulin-stimulated MAP2 kinase, Insulin stimulated MAP2 kinase, HuMKER1A, HS44KDAP, Extracellular signal-regulated kinase 1, Extracellular signal regulated kinase 1, ERT-2, ERT2, ERT 2, ERK-2, ERK2, ERK-1, ERK1, ERK 2, ERK 1, ATGP0687-10 µg, ATGP0687-20 µg, ATGP0687-50 µg, ATGP0687-100 µg, ATGP0687-250 µg, ATGP0687-500 µg, ATGP0687-1 mg, ATGP0687-010, ATGP0687-020, ATGP0687-050, ATGP0687-100, ATGP0687-250, ATGP0687-500, ATGP0687-01M

Kim DW, et al. (2000) Mol Cell Biol. 20 (4) :1140-8

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Cell Cycle

NP_002737

P27361

Human

MGSSHHHHHH SSGLVPRGSH MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS NRPIFPGKHY LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD SKALDLLDRM LTFNPNKRIT VEEALAHPYL EQYYDPTDEP VAEEPFTFAM ELDDLPKERL KELIFQETAR FQPGVLEAP