Recombinant humaan ERK2/MAPK1-eiwit

MAPK1, also known as ERK (extracellular signal-regulated kinase), acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Recombinant human MAPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

PRKM2, PRKM1, p42MAPK, p41MAPK, p41, p40, p38, Mitogen-activated protein kinase 1 MAP kinase 2, Mitogen-activated protein kinase 1, MAPK2, MAPK1, Extracellular Signal Regulated Kinase 2, ERT1, ERK2, ERK,

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

43.5 kDa (380aa) confirmed by MALDI-TOF

ERK, ERK 2, ERK2, ERT 1, ERT1, Extracellular Signal Regulated Kinase 2, MAPK 1, MAPK 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 1 MAP kinase 2, p38, p40, p41, p41mapk, P42MAPK, PRKM1, PRKM2, ATGP0445-10 µg, ATGP0445-20 µg, ATGP0445-50 µg, ATGP0445-100 µg, ATGP0445-250 µg, ATGP0445-500 µg, ATGP0445-1 mg, ATGP0445-010, ATGP0445-020, ATGP0445-050, ATGP0445-100, ATGP0445-250, ATGP0445-500, ATGP0445-01M

Hu S., et al. (2009) Cell. 139 (3) :610-22. ; ; McGargill MA., et al. (2009) J Immunol. 183 (8) :4838-42.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

Signal Transduction

NP_620407

P28482

Human

MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS