Recombinant humaan Ero1L alfa-eiwit

ERO1-like protein alpha, also known as EPO1L, is an essential oxidoreductase that oxidizes proteins and is required for the folding of immunoglobulins. EPO1L covalently binds with PDI (protein disulfide-isomerase) and together they produce disulfide bonds between proteins in the endoplasmic reticulum. EPO1L is stimulated by hypoxia, suggesting that it is regulated through the HIF (hypoxia inducible transcrip-tion factor) pathway. EPO1L is ubiquitously expressed at low levels but expressed at high levels in upper digestive tract and esophagus. Recombinant human EPO1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ERO1-like protein alpha, ERO1-alpha, ERO1A

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

54.4 kDa (468aa)

ERO1L a, ERO1-like protein alpha, ERO1-alpha, ERO1A, ATGP2669-10 µg, ATGP2669-20 µg, ATGP2669-50 µg, ATGP2669-100 µg, ATGP2669-250 µg, ATGP2669-500 µg, ATGP2669-1 mg, ATGP2669-10, ATGP2669-20, ATGP2669-50, ATGP2669-100, ATGP2669-250, ATGP2669-500, ATGP2669-1

Otsu M., et al. (2006) Antioxid. Redox Signal. 8: 274-282.; ; Gess B., et al. (2003) J Biochem. 270: 2228-2235.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Signal Transduction

NP_055399

Q96HE7

Human

MGSSHHHHHH SSGLVPRGSH MGSEEQPPET AAQRCFCQVS GYLDDCTCDV ETIDRFNNYR LFPRLQKLLE SDYFRYYKVN LKRPCPFWND ISQCGRRDCA VKPCQSDEVP DGIKSASYKY SEEANNLIEE CEQAERLGAV DESLSEETQK AVLQWTKHDD SSDNFCEADD IQSPEAEYVD LLLNPERYTG YKGPDAWKIW NVIYEENCFK PQTIKRPLNP LASGQGTSEE NTFYSWLEGL CVEKRAFYRL ISGLHASINV HLSARYLLQE TWLEKKWGHN ITEFQQRFDG ILTEGEGPRR LKNLYFLYLI ELRALSKVLP FFERPDFQLF TGNKIQDEEN KMLLLEILHE IKSFPLHFDE NSFFAGDKKE AHKLKEDFRL HFRNISRIMD CVGCFKCRLW GKLQTQGLGT ALKILFSEKL IANMPESGPS YEFHLTRQEI VSLFNAFGRI STSVKELENF RNLLQNIH