Recombinant humaan ERP44 eiwit

ERP44 is a novel endoplasmic reticulum chaperone involved in thiol-dependent retention of the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. The protein inhibits the calcium channel activity of ITPR1. This protein may have a role in the control of oxidative protein folding in the endoplasmic reticulum. Recombinant human ERP44 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Endoplasmic reticulum protein 44, PDIA10, TXNDC4, ER protein 44, Endoplasmic reticulum protein 44

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

48.0 kDa (415aa) confirmed by MALDI-TOF

ERP44, Endoplasmic reticulum protein 44, PDIA10, TXNDC4, ER protein 44, Endoplasmic reticulum protein 44, ATGP0678-10 µg, ATGP0678-20 µg, ATGP0678-50 µg, ATGP0678-100 µg, ATGP0678-250 µg, ATGP0678-500 µg, ATGP0678-1 mg, ATGP0678-10, ATGP0678-20, ATGP0678-50, ATGP0678-100, ATGP0678-250, ATGP0678-500, ATGP0678-1

Von Figura K., et al. (2008) J Biol Chem. 283 (10) :6375-83.; ; Sitia R., et al. (2002) EMBO J. 21 (4) :835-44;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Heat Shock Proteins

NP_055866

Q9BS26

Human

EI TSLDTENIDE ILNNADVALV NFYADWCRFS QMLHPIFEEA SDVIKEEFPN ENQVVFARVD CDQHSDIAQR YRISKYPTLK LFRNGMMMKR EYRGQRSVKA LADYIRQQKS DPIQEIRDLA EITTLDRSKR NIIGYFEQKD SDNYRVFERV ANILHDDCAF LSAFGDVSKP ERYSGDNIIY KPPGHSAPDM VYLGAMTNFD VTYNWIQDKC VPLVREITFE NGEELTEEGL PFLILFHMKE DTESLEIFQN EVARQLISEK GTINFLHADC DKFRHPLLHI QKTPADCPVI AIDSFRHMYV FGDFKDVLIP GKLKQFVFDL HSGKLHREFH HGPDPTDTAP GEQAQDVASS PPESSFQKLA PSEYRYTLLR DRDEL