Recombinant humaan erytropoëtine R/EPOR-eiwit

EPOR, also known as Erythropoietin receptor, mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, it dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. So it may also activate the LYN tyrosine kinase. Recombinant human EPOR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤70 ng/mL with Human Erythropoietin.

Erythropoietin receptor, EPO-R, EPOR

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤70 ng/mL with Human Erythropoietin.

25.6 kDa (232aa)

Erythropoietin R, ATGP3494-10 µg, ATGP3494-20 µg, ATGP3494-50 µg, ATGP3494-100 µg, ATGP3494-250 µg, ATGP3494-500 µg, ATGP3494-1 mg, ATGP3494-10, ATGP3494-20, ATGP3494-50, ATGP3494-100, ATGP3494-250, ATGP3494-500, ATGP3494-1

Nakamura Y., et al. (1992) Science. 257:1138-1141.; ; Kirito K., et al. (2002) Blood. 99:102-110.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_000112

P19235

Human

Ala25