Recombinant humaan erytropoëtine/EPO-eiwit

EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant human EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤0.5 ng/mL.

EPO, EP, MVCD2

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤0.5 ng/mL.

19.5 kDa (174aa)

Erythropoietin, ATGP3208-10 µg, ATGP3208-20 µg, ATGP3208-50 µg, ATGP3208-100 µg, ATGP3208-250 µg, ATGP3208-500 µg, ATGP3208-1 mg, ATGP3208-10, ATGP3208-20, ATGP3208-50, ATGP3208-100, ATGP3208-250, ATGP3208-500, ATGP3208-1

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263.; ; Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Angiogenesis

NP_000790.1

P01588

Human