Recombinant humaan ETFB-eiwit

ETF (electron transfer flavoprotein) is a heterodimer of an alpha and beta subunit. The ETFB protein is electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Recombinant human ETFB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Electron transfer flavoprotein subunit beta, FP585, MADD

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30 kDa (275aa) confirmed by MALDI-TOF

ETFB, Electron transfer flavoprotein subunit beta, FP585, MADD, ATGP1047-10 µg, ATGP1047-20 µg, ATGP1047-50 µg, ATGP1047-100 µg, ATGP1047-250 µg, ATGP1047-500 µg, ATGP1047-1 mg, ATGP1047-10, ATGP1047-20, ATGP1047-50, ATGP1047-100, ATGP1047-250, ATGP1047-500, ATGP1047-1

Colombo I., et al. (1994) Hum. Mol. Genet. 3:429-435; ; Bross P., et al. (1999) Mol. Genet. Metab. 67:138-147

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl

Metabolism

NP_001976

P38117

Human

MGSSHHHHHH SSGLVPRGSH MAELRVLVAV KRVIDYAVKI RVKPDRTGVV TDGVKHSMNP FCEIAVEEAV RLKEKKLVKE VIAVSCGPAQ CQETIRTALA MGADRGIHVE VPPAEAERLG PLQVARVLAK LAEKEKVDLV LLGKQAIDDD CNQTGQMTAG FLDWPQGTFA SQVTLEGDKL KVEREIDGGL ETLRLKLPAV VTADLRLNEP RYATLPNIMK AKKKKIEVIK PGDLGVDLTS KLSVISVEDP PQRTAGVKVE TTEDLVAKLK EIGRI