Recombinant humaan EXOSC1-eiwit

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of Au rich element-containing RNAs but not for poly (A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Recombinant human EXOSC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Exosome complex component CSL4, Exosome component 1, CGI-108, CSL4, Csl4p, hCsl4p, p13, RP11-452K12.9, SKI4, Ski4p

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.8 kDa (218aa) confirmed by MALDI-TOF

EXOSC1, Exosome complex component CSL4, Exosome component 1, CGI-108, CSL4, Csl4p, hCsl4p, p13, RP11-452K12.9, SKI4, Ski4p, ATGP2723-10 µg, ATGP2723-20 µg, ATGP2723-50 µg, ATGP2723-100 µg, ATGP2723-250 µg, ATGP2723-500 µg, ATGP2723-1 mg, ATGP2723-10, ATGP2723-20, ATGP2723-50, ATGP2723-100, ATGP2723-250, ATGP2723-500, ATGP2723-1

Lai C.-H., Chou C.-Y., et al. (2000) Genome Res. 10:703-713

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_057130

Q9Y3B2

Human

MGSSHHHHHH SSGLVPRGSH MGSMAPPVRY CIPGERLCNL EEGSPGSGTY TRHGYIFSSL AGCLMKSSEN GALPVVSVVR ETESQLLPDV GAIVTCKVSS INSRFAKVHI LYVGSMPLKN SFRGTIRKED VRATEKDKVE IYKSFRPGDI VLAKVISLGD AQSNYLLTTA ENELGVVVAH SESGIQMVPI SWCEMQCPKT HTKEFRKVAR VQPEFLQT