Recombinant humaan EXOSC3-eiwit

EXOSC3 is a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21. Alternatively spliced transcript variants encoding different isoforms have been described. Recombinant human EXOSC3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Exosome complex component RRP40 isoform 1, bA3J10.7, CGI-102, hRrp-40, p10, PCH1B, RP11-3J10.8, RRP40, Rrp40p

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.0 kDa (298aa) confirmed by MALDI-TOF

EXOSC3, exosome complex component RRP40 isoform 1, bA3J10.7, CGI-102, hRrp-40, p10, PCH1B, RP11-3J10.8, RRP40, Rrp40p, ATGP2703-10 µg, ATGP2703-20 µg, ATGP2703-50 µg, ATGP2703-100 µg, ATGP2703-250 µg, ATGP2703-500 µg, ATGP2703-1 mg, ATGP2703-10, ATGP2703-20, ATGP2703-50, ATGP2703-100, ATGP2703-250, ATGP2703-500, ATGP2703-1

Mukherjee D., et al (2002) . EMBO J. 21:165-174; ; van Dijk E.L., et al (2007) . RNA 13:1027-1035;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_057126

Q9NQT5

Human

MAEPASV AAESLAGSRA RAARTVLGQV VLPGEELLLP EQEDAEGPGG AVERPLSLNA RACSRVRVVC GPGLRRCGDR LLVTKCGRLR HKEPGSGSGG GVYWVDSQQK RYVPVKGDHV IGIVTAKSGD IFKVDVGGSE PASLSYLSFE GATKRNRPNV QVGDLIYGQF VVANKDMEPE MVCIDSCGRA NGMGVIGQDG LLFKVTLGLI RKLLAPDCEI IQEVGKLYPL EIVFGMNGRI WVKAKTIQQT LILANILEAC EHMTSDQRKQ IFSRLAES