Recombinant humaan EXOSC5-eiwit

EXOSC5, also known as exosome component 5, belongs to the RNase PH family. EXOSC5 has been shown to interact with Exosome component 8 and Exosome component 1. It is Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. It highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis. Recombinant human EXOSC5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Exosome complex component RRP46, hRrp46p, p12B, RRP41B, RRP46, Rrp46p

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.5 kDa (256aa) confirmed by MALDI-TOF

EXOSC5, Exosome complex component RRP46, hRrp46p, p12B, RRP41B, RRP46, Rrp46p, ATGP1458-10 µg, ATGP1458-20 µg, ATGP1458-50 µg, ATGP1458-100 µg, ATGP1458-250 µg, ATGP1458-500 µg, ATGP1458-1 mg, ATGP1458-10, ATGP1458-20, ATGP1458-50, ATGP1458-100, ATGP1458-250, ATGP1458-500, ATGP1458-1

Mukherjee D., et al. (2002) EMBO J. 21:165-174; ; Basu u., et al. (2011) Cell 144:353-363

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 2mM DTT, 30% glycerol, 200 mM NaCl

Epigenomics (Transcription & Translation)

NP_064543.3

Q9NQT4

Human

MEEETHTDA KIRAENGTGS SPRGPGCSLR HFACEQNLLS RPDGSASFLQ GDTSVLAGVY GPAEVKVSKE IFNKATLEVI LRPKIGLPGV AEKSRERLIR NTCEAVVLGT LHPRTSITVV LQVVSDAGSL LACCLNAACM ALVDAGVPMR ALFCGVACAL DSDGTLVLDP TSKQEKEARA VLTFALDSVE RKLLMSSTKG LYSDTELQQC LAAAQAASQH VFRFYRESLQ RRYSKS