Recombinant humaan FAAH2-eiwit

FAAH2 is a fatty acid amide hydrolase that shares a conserved protein motif with the amidase signature family of enzymes. The encoded enzyme is able to catalyze the hydrolysis of a broad range of bioactive lipids, including those from the three main classes of fatty acid amides; N-acylethanolamines, fatty acid primary amides and N-acyl amino acids. This enzyme has a preference for monounsaturated acyl chains as a substrate. Recombinant human FAAH2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Fatty acid amide hydrolase 2, AMDD, Amidase domain containing, Anandamide amidohydrolase 2, Oleamide hydrolase 2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

57.4 kDa (524aa)

AMDD, Amidase domain containing, Anandamide amidohydrolase 2, FAA, Fatty acid amide hydrolase 2, Fatty-acid amide hydrolase 2, Oleamide hydrolase 2, ATGP2459-10 µg, ATGP2459-20 µg, ATGP2459-50 µg, ATGP2459-100 µg, ATGP2459-250 µg, ATGP2459-500 µg, ATGP2459-1 mg, ATGP2459-010, ATGP2459-020, ATGP2459-050, ATGP2459-100, ATGP2459-250, ATGP2459-500, ATGP2459-01M

Wei B.Q., Mikkelsen T.S.. et al. (2006) . J. Biol. Chem. 281:36569-36578

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing , 10% glycerol, 0.4M urea

Metabolism

NP_777572

Q6GMR7

Human

MGSSHHHHHH SSGLVPRGSH MGSGGPKFAS KTPRPVTEPL LLLSGMQLAK LIRQRKVKCI DVVQAYINRI KDVNPMINGI VKYRFEEAMK EAHAVDQKLA EKQEDEATLE NKWPFLGVPL TVKEAFQLQG MPNSSGLMNR RDAIAKTDAT VVALLKGAGA IPLGITNCSE LCMWYESSNK IYGRSNNPYD LQHIVGGSSG GEGCTLAAAC SVIGVGSDIG GSIRMPAFFN GIFGHKPSPG VVPNKGQFPL AVGAQELFLC TGPMCRYAED LAPMLKVMAG PGIKRLKLDT KVHLKDLKFY WMEHDGGSFL MSKVDQDLIM TQKKVVVHLE TILGASVQHV KLKKMKYSFQ LWIAMMSAKG HDGKEPVKFV DLLGDHGKHV SPLWELIKWC LGLSVYTIPS IGLALLEEKL RYSNEKYQKF KAVEESLRKE LVDMLGDDGV FLYPSHPTVA PKHHVPLTRP FNFAYTGVFS ALGLPVTQCP LGLNAKGLPL GIQVVAGPFN DHLTLAVAQY LEKTFGGWVC PGKF