Recombinant humaan FABP2/I-FABP-eiwit

FABP2, also known as intestinal fatty acid binding protein 2 (I-FABP), is expressed in the epithelium of the small intestine by mature enterocytes. FABP2 is thought to facilitate of cellular uptake and transport of long-chain fatty acids within enterocytes and may also help maintain energy homeostasis by functioning as a lipid sensor. This protein binds saturated long-chain fatty acid with a high affinity, but binds with a low affinity to unsaturated long-chain fatty acid. The Ala to Thr substitution at residue 54 of FABP2 is associated with higher total cholesterol, with stroke incidence, elevation of fasting and postprandial triglyceride, insulin resistance, and higher nonesterified fatty acid (NEFA) concentrations. Recombinant human FABP2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Intestinal fatty acid binding protein 2, Intestinal fatty acid binding protein 2, FABPI, I-FABP, Intestinal fatty acid binding protein 2 FABP 2, FABP2, Fatty acid binding protein, Fatty acid binding protein 2 intestinal, Fatty acid binding protein intestinal, I FABP, IFABP, Intestinal fatty acid binding protein 2, MGC133132, Fatty Acid Binding Protein-2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

17.3 kDa (152aa) confirmed by MALDI-TOF

FABP2, I-FABP, Intestinal fatty acid binding protein 2, Intestinal fatty acid binding protein 2, FABPI, I-FABP, Intestinal fatty acid binding protein 2 FABP 2, FABP2, Fatty acid binding protein, Fatty acid binding protein 2 intestinal, Fatty acid binding protein intestinal, I FABP, IFABP, Intestinal fatty acid binding protein 2, MGC133132, Fatty Acid Binding Protein-2, ATGP0300-10 µg, ATGP0300-20 µg, ATGP0300-50 µg, ATGP0300-100 µg, ATGP0300-250 µg, ATGP0300-500 µg, ATGP0300-1 mg, ATGP0300-10, ATGP0300-20, ATGP0300-50, ATGP0300-100, ATGP0300-250, ATGP0300-500, ATGP0300-1

Baier LJ., et al. (1995) . J Clin Invest. 95 (3) :1281-7. ; ; Helwig u., et al. (2007) Metabolism. 56 (6) :723-31. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Transportation

NP_000125

P12104

Human

MGSSHHHHHH SSGLVPRGSH MAFDSTWKVD RSENYDKFME KMGVNIVKRK LAAHDNLKLT ITQEGNKFTV KESSAFRNIE VVFELGVTFN YNLADGTELR GTWSLEGNKL IGKFKRTDNG NELNTVREII GDELVQTYVY EGVEAKRIFK KD