Recombinant humaan FAHD1-eiwit

Fumarylacetoacetate hydrolase domain-containing protein 1, also known as FAHD1, belongs to the FAH family. FAHD1 is thought to have hydrolase activity and uses Magnesium and Calcium as cofactors. It appears highly probable that the metal binding sites are involved in an enzymatic activity related to the catabolism of aromatic amino acids. Recombinant human FAHD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Fumarylacetoacetate hydrolase domain-containing protein 1, YISKL

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

27 kDa (244aa) confirmed by MALDI-TOF

FAHD1, Fumarylacetoacetate hydrolase domain-containing protein 1, YISKL, ATGP0904-10 µg, ATGP0904-20 µg, ATGP0904-50 µg, ATGP0904-100 µg, ATGP0904-250 µg, ATGP0904-500 µg, ATGP0904-1 mg, ATGP0904-10, ATGP0904-20, ATGP0904-50, ATGP0904-100, ATGP0904-250, ATGP0904-500, ATGP0904-1

Naniasetty BA., et al. (2004) Biol chem. 385 (10) :935-42.; ; Wiemann S., et al. (2001) Genome Res. 11 (3) :422-35.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_112485

Q6P587

Human

MGIMAASRPL SRFWEWGKNI VCVGRNYADH VREMRSAVLS EPVLFLKPST AYAPEGSPIL MPAYTRNLHH ELELGVVMGK RCRAVPEAAA MDYVGGYALC LDMTARDVQD ECKKKGLPWT LAKSFTASCP VSAFVPKEKI PDPHKLKLWL KVNGELRQEG ETSSMIFSIP YIISYVSKII TLEEGDIILT GTPKGVGPVK ENDEIEAGIH GLVSMTFKVE KPEY