Recombinant humaan FAM84B/LRATD2-eiwit

FAM84B, as known as Family with sequence similarity 84, member B, coprecipitated with a downstream effector of RAS, CRAF. Binding of FAM83B with CRAF disrupted CRAF/14-3-3 interactions and increased CRAF membrane localization, resulting in elevated MAPK and mammalian target of rapamycin (mTOR) signaling. It is an oncogene and potentially represents a new target for therapeutic intervention. Recombinant human FAM84B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

LRAT domain containing 2, Family with sequence similarity 84 member B, BCMP101, NSE2, Breast cancer membrane-associated protein 101, Neurological/sensory 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.9 kDa (333aa) confirmed by MALDI-TOF

FAM84B, Family with sequence similarity 84 member B, Family with sequence similarity 84, member B, BCMP101, NSE2, ATGP2442-10 µg, ATGP2442-20 µg, ATGP2442-50 µg, ATGP2442-100 µg, ATGP2442-250 µg, ATGP2442-500 µg, ATGP2442-1 mg, ATGP2442-10, ATGP2442-20, ATGP2442-50, ATGP2442-100, ATGP2442-250, ATGP2442-500, ATGP2442-1

Kraja AT. et al. (2013) Lipids. 48:155-165; ; Camps J. et al. (2009) Genes Chromosomes Cancer. 48:1002-1017.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl,1mM DTT, 0.1mM PMSF

Neuroscience

NP_777571

Q96KN1

Human

MGNQVEK LTHLSYKEVP TADPTGVDRD DGPRIGVSYI FSNDDEDVEP QPPPQGPDGG GLPDGGDGPP PPQPQPYDPR LHEVECSVFY RDECIYQKSF APGSAALSTY TPENLLNKCK PGDLVEFVSQ AQYPHWAVYV GNFQVVHLHR LEVINSFLTD ASQGRRGRVV NDLYRYKPLS SSAVVRNALA HVGAKERELS WRNSESFAAW CRYGKREFKI GGELRIGKQP YRLQIQLSAQ RSHTLEFQSL EDLIMEKRRN DQIGRAAVLQ ELATHLHPAE PEEGDSNVAR TTPPPGRPPA PSSEEEDGEA VAH