Recombinant humaan FARS2-eiwit

Phenylalanyl-tRNA synthetase 2, mitochondrial (PheRS), also known as FARS2, belongs to the class II aminoacyl- tRNA synthetase family. FARS2 is a mitochondrial matrix protein. Functioning as a monomer, FARS2 catalyzes the ATP-dependent conversion of L-phenylalanine and tRNA (Phe) to L-phenlalanyltRNA (Phe), an event that is crucial for proper translation and protein expression. Recombinant human FARS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phenylalanyl-tRNA synthetase 2 mitochondrial, Phenylalanyl-tRNA synthetase 2, mitochondrial, dJ520B18.2, FARS1, HSPC320, PheRS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

50.6 kDa (436aa) confirmed by MALDI-TOF

FARS2, ATGP1015-10 µg, ATGP1015-20 µg, ATGP1015-50 µg, ATGP1015-100 µg, ATGP1015-250 µg, ATGP1015-500 µg, ATGP1015-1 mg, ATGP1015-10, ATGP1015-20, ATGP1015-50, ATGP1015-100, ATGP1015-250, ATGP1015-500, ATGP1015-1

Sasaki H M., et al. (2006) Proc Natl Acad Sci uSA. 103:14744-14749.; ; Bullard J M., et al. (1999) J Mol Biol. 288:567-577.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 1mM EDTA, 0.1M NaCl

Metabolism

NP_006558.1

O95363

Human

PAAECATQR APGSVVELLG KSYPQDDHSN LTRKVLTRVG RNLHNQQHHP LWLIKERVKE HFYKQYVGRF GTPLFSVYDN LSPVVTTWQN FDSLLIPADH PSRKKGDNYY LNRTHMLRAH TSAHQWDLLH AGLDAFLVVG DVYRRDQIDS QHYPIFHQLE AVRLFSKHEL FAGIKDGESL QLFEQSSRSA HKQETHTMEA VKLVEFDLKQ TLTRLMAHLF GDELEIRWVD CYFPFTHPSF EMEINFHGEW LEVLGCGVME QQLVNSAGAQ DRIGWAFGLG LERLAMILYD IPDIRLFWCE DERFLKQFCV SNINQKVKFQ PLSKYPAVIN DISFWLPSEN YAENDFYDLV RTIGGDLVEK VDLIDKFVHP KTHKTSHCYR ITYRHMERTL SQREVRHIHQ ALQEAAVQLL GVEGRF