Recombinant humaan FBP2-eiwit

FBP2 belongs to the FBPase class 1 family. The protein is a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Recombinant human FBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

39 kDa (362aa) confirmed by MALDI-TOF

FBP2, Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2, ATGP1808-10 µg, ATGP1808-20 µg, ATGP1808-50 µg, ATGP1808-100 µg, ATGP1808-250 µg, ATGP1808-500 µg, ATGP1808-1 mg, ATGP1808-10, ATGP1808-20, ATGP1808-50, ATGP1808-100, ATGP1808-250, ATGP1808-500, ATGP1808-1

Gizak A, Maciaszczyk E, et al. (2008) . Proteins. 72 (1) :209-16.; ; Rakus D, Maciaszczyk E, et al. (2005) . FEBS Lett. 579 (25) :5577-81.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_003828

O00757

Human

MTDRSPF ETDMLTLTRY VMEKGRQAKG TGELTQLLNS MLTAIKAISS AVRKAGLAHL YGIAGSVNVT GDEVKKLDVL SNSLVINMVQ SSYSTCVLVS EENKDAIITA KEKRGKYVVC FDPLDGSSNI DCLASIGTIF AIYRKTSEDE PSEKDALQCG RNIVAAGYAL YGSATLVALS TGQGVDLFML DPALGEFVLV EKDVKIKKKG KIYSLNEGYA KYFDAATTEY VQKKKFPEDG SAPYGARYVG SMVADVHRTL VYGGIFLYPA NQKSPKGKLR LLYECNPVAY IIEQAGGLAT TGTQPVLDVK PEAIHQRVPL ILGSPEDVQE YLTCVQKNQA GS