Recombinant humaan FBPase 1-eiwit

Fructose-1, 6-bisphosphatase 1 (FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

FBPase1, FBP, FBP1, D fructose 1 6 bisphosphate 1 phosphohydrolase 1, EC 3.1.3.11, FBP 1, FBPase 1, Fructose 1 6 bisphosphatase 1 Fructose bisphosphatase 1, Growth inhibiting protein 17, Liver fructose bisphosphatase.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

39.0 kDa (358aa) confirmed by MALDI-TOF

FBPase1, FBPase1, FBP, FBP1, D fructose 1 6 bisphosphate 1 phosphohydrolase 1, EC 3.1.3.11, FBP 1, FBPase 1, Fructose 1 6 bisphosphatase 1 Fructose bisphosphatase 1, Growth inhibiting protein 17, Liver fructose bisphosphatase., ATGP0408-10 µg, ATGP0408-20 µg, ATGP0408-50 µg, ATGP0408-100 µg, ATGP0408-250 µg, ATGP0408-500 µg, ATGP0408-1 mg, ATGP0408-10, ATGP0408-20, ATGP0408-50, ATGP0408-100, ATGP0408-250, ATGP0408-500, ATGP0408-1

Rabenhorst u., et al. (2009) Hepatology. 50 (4) :1121-9. ; ; Hirota K., et al. (2008) Nature. 456 (7218) :130-4.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_000498.2

P09467

Human

MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ