Recombinant humaan FBPase 1-eiwit

Fructose-1, 6-bisphosphatase 1 (FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 7,000 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.

FBPase1, FBP, FBP1

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 7,000pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.

39.0 kDa (358aa) confirmed by MALDI-TOF

FBPase1, FBPase1, FBP, FBP1, ATGP3234-10 µg, ATGP3234-20 µg, ATGP3234-50 µg, ATGP3234-100 µg, ATGP3234-250 µg, ATGP3234-500 µg, ATGP3234-1 mg, ATGP3234-10, ATGP3234-20, ATGP3234-50, ATGP3234-100, ATGP3234-250, ATGP3234-500, ATGP3234-1

Rabenhorst U., et al. (2009) Hepatology. 50 (4) :1121-9. ; ; Hirota K., et al. (2008) Nature. 456 (7218) :130-4.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Metabolism

NP_000498.2

P09467

Human

MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ