Recombinant humaan FBXO2-eiwit

FBXO2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein belongs to the Fbxs class. It is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state. Recombinant human FBXO2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

F-box only protein 2, FBG1, Fbs1, FBX2, NFB42, OCP1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

35.7 kDa (319aa) confirmed by MALDI-TOF

FBXO2, F-box only protein 2, FBG1, Fbs1, FBX2, NFB42, OCP1, ATGP2752-10 µg, ATGP2752-20 µg, ATGP2752-50 µg, ATGP2752-100 µg, ATGP2752-250 µg, ATGP2752-500 µg, ATGP2752-1 mg, ATGP2752-10, ATGP2752-20, ATGP2752-50, ATGP2752-100, ATGP2752-250, ATGP2752-500, ATGP2752-1

Cenciarelli C., et al (1999) . Curr. Biol. 9:1177-1179; ; Burkard T.R., et al (2011) . BMC Syst. Biol. 5:17-17;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Ubiquitination

NP_036300

Q9UK22

Human

MGSSHHHHHH SSGLVPRGSH MGSMDGDGDP ESVGQPEEAS PEEQPEEASA EEERPEDQQE EEAAAAAAYL DELPEPLLLR VLAALPAAEL VQACRLVCLR WKELVDGAPL WLLKCQQEGL VPEGGVEEER DHWQQFYFLS KRRRNLLRNP CGEEDLEGWC DVEHGGDGWR VEELPGDSGV EFTHDESVKK YFASSFEWCR KAQVIDLQAE GYWEELLDTT QPAIVVKDWY SGRSDAGCLY ELTVKLLSEH ENVLAEFSSG QVAVPQDSDG GGWMEISHTF TDYGPGVRFV RFEHGGQDSV YWKGWFGARV TNSSVWVEP