Recombinant humaan FBXO6-eiwit

FBXO6 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO6 belongs to the Fbxs class, and its C-terminal region is highly similar to that of rat NFB42 (neural F Box 42 kDa) which may be involved in the control of the cell cycle. Recombinant human FBXO6 protein, fused to His-tag at N-terminus, was expressed in E. coli.

F-box only protein 6, FBG2, FBS2, FBX6, Fbx6b

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.3 kDa (316aa)

FBXO6, F-box only protein 6, FBG2, FBS2, FBX6, Fbx6b, ATGP2168-10 µg, ATGP2168-20 µg, ATGP2168-50 µg, ATGP2168-100 µg, ATGP2168-250 µg, ATGP2168-500 µg, ATGP2168-1 mg, ATGP2168-10, ATGP2168-20, ATGP2168-50, ATGP2168-100, ATGP2168-250, ATGP2168-500, ATGP2168-1

Ceniarelli C., et al. (1998) Curr Biol. 21; ;9 (20) :1177-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Ubiquitination

NP_060908

Q9NRD1

Human

MDAPHSK AALDSINELP ENILLELFTH VPARQLLLNC RLVCSLWRDL IDLMTLWKRK CLREGFITKD WDQPVADWKI FYFLRSLHRN LLRNPCAEED MFAWQIDFNG GDRWKVESLP GAHGTDFPDP KVKKYFVTSY EMCLKSQLVD LVAEGYWEEL LDTFRPDIVV KDWFAARADC GCTYQLKVQL ASADYFVLAS FEPPPVTIQQ WNNATWTEVS YTFSDYPRGV RYILFQHGGR DTQYWAGWYG PRVTNSSIVV SPKMTRNQAS SEAQPGQKHG QEEAAQSPYR AVVQIF