Recombinant humaan FDFT1-eiwit

FDFT1 is a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. Recombinant human FDFT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Squalene synthase, DGPT, ERG9, SQS, SS

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.4 kDa (306aa)

FDFT1, Squalene synthase, DGPT, ERG9, SQS, SS, ATGP2789-10 µg, ATGP2789-20 µg, ATGP2789-50 µg, ATGP2789-100 µg, ATGP2789-250 µg, ATGP2789-500 µg, ATGP2789-1 mg, ATGP2789-10, ATGP2789-20, ATGP2789-50, ATGP2789-100, ATGP2789-250, ATGP2789-500, ATGP2789-1

Tansey TR, Shechter I (2000) Biochim. Biophys. Acta 1529 (1-3) : 49-62.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Metabolism

NP_004453

P37268

Human

MGSSHHHHHH SSGLVPRGSH MGSMEFVKCL GHPEEFYNLV RFRIGGKRKV MPKMDQDSLS SSLKTCYKYL NQTSRSFAAV IQALDGEMRN AVCIFYLVLR ALDTLEDDMT ISVEKKVPLL HNFHSFLYQP DWRFMESKEK DRQVLEDFPT ISLEFRNLAE KYQTVIADIC RRMGIGMAEF LDKHVTSEQE WDKYCHYVAG LVGIGLSRLF SASEFEDPLV GEDTERANSM GLFLQKTNII RDYLEDQQGG REFWPQEVWS RYVKKLGDFA KPENIDLAVQ CLNELITNAL HHIPDVITYL SRLRNQ