Recombinant humaan FEN1-eiwit

FEN-1, also known as Flap structure-specific endonuclease 1, removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication. Recombinant human FEN-1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Flap structure-specific endonuclease 1, FEN-1, MF1, RAD2, Flap structure-specific endonuclease 1 DNase IV, Flap endonuclease 1, Flap structure specific endonuclease 1, hFEN1, Maturation factor 1

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42.5 kDa (380aa) confirmed by MALDI-TOF

FEN1, Flap structure-specific endonuclease 1, FEN-1, MF1, RAD2, Flap structure-specific endonuclease 1 DNase IV, Flap endonuclease 1, Flap structure specific endonuclease 1, hFEN1, Maturation factor 1, ATGP0442-10 µg, ATGP0442-20 µg, ATGP0442-50 µg, ATGP0442-100 µg, ATGP0442-250 µg, ATGP0442-500 µg, ATGP0442-1 mg, ATGP0442-10, ATGP0442-20, ATGP0442-50, ATGP0442-100, ATGP0442-250, ATGP0442-500, ATGP0442-1

Hakoyama T., et al. (2009) Nature. 462 (7272) :514-7. ; ; Lal A., et al. (2009) Mol Cell. 35 (5) :610-25.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol

Enzymes & Proteases

NP_004102.1

P39748

Human

MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK